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J Forensic Sci. 2013 Jul;58(4):989-92. doi: 10.1111/1556-4029.12166. Epub 2013 May 17.

High-throughput STR analysis for DNA database using direct PCR.

Author information

1
DNA Analysis Laboratory, Division of Forensic DNA, Supreme Prosecutors' Office, Seoul, 137-730, Korea.

Abstract

Since the Korean criminal DNA database was launched in 2010, we have focused on establishing an automated DNA database profiling system that analyzes short tandem repeat loci in a high-throughput and cost-effective manner. We established a DNA database profiling system without DNA purification using a direct PCR buffer system. The quality of direct PCR procedures was compared with that of conventional PCR system under their respective optimized conditions. The results revealed not only perfect concordance but also an excellent PCR success rate, good electropherogram quality, and an optimal intra/inter-loci peak height ratio. In particular, the proportion of DNA extraction required due to direct PCR failure could be minimized to <3%. In conclusion, the newly developed direct PCR system can be adopted for automated DNA database profiling systems to replace or supplement conventional PCR system in a time- and cost-saving manner.

KEYWORDS:

Korean DNA database; buccal FTA card; direct polymerase chain reaction; forensic science; high-throughput; offender; short tandem repeat

PMID:
23683293
DOI:
10.1111/1556-4029.12166
[Indexed for MEDLINE]

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