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Methods Mol Biol. 2013;1018:301-11. doi: 10.1007/978-1-62703-444-9_28.

Cryosectioning.

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1
Department of Chemical Biology, Susan Lehman Cullman Laboratory for Cancer Research, Ernest Mario School of Pharmacy, Rutgers University, Piscataway, NJ, USA.

Abstract

Cryosectioning, the sectioning of frozen specimens, has been an important histological tool for more than a century and continues to be extensively utilized today. However, the ability to produce high-quality sections is often a difficult process requiring extensive patience and experience. In this chapter, we have detailed an effective method for the embedding, mounting, and sectioning of frozen tissues, as well as have provided suggestions in producing high-quality sections.

PMID:
23681639
DOI:
10.1007/978-1-62703-444-9_28
[Indexed for MEDLINE]
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