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PLoS One. 2013 May 10;8(5):e64454. doi: 10.1371/journal.pone.0064454. Print 2013.

Generation of murine sympathoadrenergic progenitor-like cells from embryonic stem cells and postnatal adrenal glands.

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1
Department of Pediatrics and Adolescent Medicine, University Medical Center Ulm, Ulm, Germany.

Abstract

Sympathoadrenergic progenitor cells (SAPs) of the peripheral nervous system (PNS) are important for normal development of the sympathetic PNS and for the genesis of neuroblastoma, the most common and often lethal extracranial solid tumor in childhood. However, it remains difficult to isolate sufficient numbers of SAPs for investigations. We therefore set out to improve generation of SAPs by using two complementary approaches, differentiation from murine embryonic stem cells (ESCs) and isolation from postnatal murine adrenal glands. We provide evidence that selecting for GD2 expression enriches for ESC-derived SAP-like cells and that proliferating SAP-like cells can be isolated from postnatal adrenal glands of mice. These advances may facilitate investigations about the development and malignant transformation of the sympathetic PNS.

PMID:
23675538
PMCID:
PMC3651195
DOI:
10.1371/journal.pone.0064454
[Indexed for MEDLINE]
Free PMC Article
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