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Mol Cell Proteomics. 2013 Aug;12(8):2293-312. doi: 10.1074/mcp.M112.024075. Epub 2013 May 14.

Mapping the extracellular and membrane proteome associated with the vasculature and the stroma in the embryo.

Author information

1
INSERM, U1029, 33405 Talence, France.

Abstract

In order to map the extracellular or membrane proteome associated with the vasculature and the stroma in an embryonic organism in vivo, we developed a biotinylation technique for chicken embryo and combined it with mass spectrometry and bioinformatic analysis. We also applied this procedure to implanted tumors growing on the chorioallantoic membrane or after the induction of granulation tissue. Membrane and extracellular matrix proteins were the most abundant components identified. Relative quantitative analysis revealed differential protein expression patterns in several tissues. Through a bioinformatic approach, we determined endothelial cell protein expression signatures, which allowed us to identify several proteins not yet reported to be associated with endothelial cells or the vasculature. This is the first study reported so far that applies in vivo biotinylation, in combination with robust label-free quantitative proteomics approaches and bioinformatic analysis, to an embryonic organism. It also provides the first description of the vascular and matrix proteome of the embryo that might constitute the starting point for further developments.

PMID:
23674615
PMCID:
PMC3734586
DOI:
10.1074/mcp.M112.024075
[Indexed for MEDLINE]
Free PMC Article

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