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Cell Cycle. 2013 Jun 15;12(12):1922-7. doi: 10.4161/cc.24944. Epub 2013 May 13.

α-Fucosidase as a novel convenient biomarker for cellular senescence.

Author information

1
Interfaculty Institute for Biochemistry, University of Tübingen, Tübingen, Germany.

Abstract

Due to its role in aging and antitumor defense, cellular senescence has recently attracted increasing interest. However, there is currently no single specific marker that can unequivocally detect senescent cells. Here, we identified α-L-fucosidase (α-Fuc) as a novel sensitive biomarker for cellular senescence. Regardless of the stress stimulus and cell type, α-Fuc activity was induced in all canonical types of cellular senescence, including replicative, DNA damage- and oncogene-induced senescence. Strikingly, in most models the degree of α-Fuc upregulation was higher than the induction of senescence-associated β-galactosidase (SA-β-Gal), the current gold standard for senescence detection. As α-Fuc is convenient and easy to measure, we suggest its utility as a valuable marker, in particular in cells with low SA-β-Gal activity.

KEYWORDS:

biomarker; cellular senescence; lysosome; α-fucosidase; β-galactosidase; β-glucuronidase

PMID:
23673343
PMCID:
PMC3735706
DOI:
10.4161/cc.24944
[Indexed for MEDLINE]
Free PMC Article
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