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Int J Cosmet Sci. 2013 Oct;35(5):472-6. doi: 10.1111/ics.12067. Epub 2013 Jun 27.

N-Acetylglucosamine modulates function of the skin fibroblasts.

Author information

1
Department of Pathophysiology, Poznan University of Medical Sciences, Poznan, Poland.

Abstract

BACKGROUND:

Fibroblasts are an important component of the skin determining its properties. N-Acetylglucosamine (NAG) is the substrate for hyaluronan synthesis, and it also has anti-inflammatory and anti-senescent activity in mesothelial cells.

METHODS:

We tested in in vitro-cultured human skin fibroblasts how supplementation of culture medium with NAG 10 mmol L(-1) changes properties of these cells.

RESULTS:

Fibroblasts cultured in presence of NAG produced more proteins and that was mainly due to increased synthesis of collagen (+33% vs. control, P < 0.05). Hyaluronan synthesis was increased (+107% vs. control, P < 0.001), but interleukin-6 synthesis was reduced (-22% vs. control, P < 0.05). Fibroblasts cultured in medium with NAG 10 mmol L(-1) demonstrated improved ability to heal the injured layer of cells (+34% vs. control, P < 0.05). Additionally senescence of fibroblasts undergoing replicative ageing in the presence of NAG was less pronounced, as reflected by smaller increase in the population doubling time (-70% vs. control, P < 0.05).

CONCLUSION:

We conclude that NAG induced changes in the skin fibroblasts' properties maybe important for prevention of the age-dependent changes in its structure and function.

KEYWORDS:

N-Acetylglucosamine; collagen; dermal fibroblasts; hyaluronan; inflammation; senescence

PMID:
23672714
DOI:
10.1111/ics.12067
[Indexed for MEDLINE]

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