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PLoS One. 2013 May 8;8(5):e63890. doi: 10.1371/journal.pone.0063890. Print 2013.

Expression of SLP-2 was associated with invasion of esophageal squamous cell carcinoma.

Author information

1
Key Laboratory of Cancer Prevention and Therapy, Department of Pathology, Tianjin Medical University Cancer Institute and Hospital, Tianjin, China.

Abstract

INTRODUCTION:

Stomatin-like protein 2 (SLP-2), a member of the Stomatin superfamily, has been identified as an oncogenic-related protein and found to be up-regulated in multi-cancers. Nonetheless, the expression pattern and regulation of SLP-2 in human esophageal squamous cell carcinoma (ESCC) remain unexplored.

METHODS:

Immunohistochemistry and immunofluorescence staining analysis were performed to show SLP-2 expression and location. RNAi method was used to inhibit specific protein expression. Transwell assay was done to investigate cells invasive capability. RT-PCR and Western blot analysis were used to detect mRNA and protein expression levels.

RESULTS:

Immunohistochemical analysis showed that up-regulation of SLP-2 was found in invasive front compared with cancer central tissue in ESCC. Inhibition of SLP-2 by SLP-2 siRNA can decrease ESCC cells invasive capability through MMP-2 dependent manner. Up-regulation of SLP-2 was effectively abrogated by the ERK1/2 inhibitors either PD98059 or U0126, but no effect was showed by the treatment of AKT inhibitors either LY294002 or MK-2206. So the regulation of SLP-2 was involved in activation of the MAPK/ERK pathway.

CONCLUSIONS:

We found that PMA/EGF could induce the up-regulated expression of SLP-2 probably through activating ERK signalling. The current study suggests that SLP-2 may represent an important molecular hallmark that is clinically relevant to the invasion of ESCC.

PMID:
23667687
PMCID:
PMC3648510
DOI:
10.1371/journal.pone.0063890
[Indexed for MEDLINE]
Free PMC Article
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