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Mol Cell. 2013 Jun 6;50(5):749-61. doi: 10.1016/j.molcel.2013.04.007. Epub 2013 May 9.

A transcriptome-wide RNAi screen in the Drosophila ovary reveals factors of the germline piRNA pathway.

Author information

1
Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA. czech@cshl.edu

Abstract

The Drosophila piRNA pathway provides an RNA-based immune system that defends the germline genome against selfish genetic elements. Two interrelated branches of the piRNA system exist: somatic cells that support oogenesis only employ Piwi, whereas germ cells utilize a more elaborate pathway centered on the three gonad-specific Argonaute proteins (Piwi, Aubergine, and Argonaute 3). While several key factors of each branch have been identified, our current knowledge is insufficient to explain the complex workings of the piRNA machinery. Here, we report a reverse genetic screen spanning the ovarian transcriptome in an attempt to uncover the full repertoire of genes required for piRNA-mediated transposon silencing in the female germline. Our screen reveals key factors of piRNA-mediated transposon silencing, including the piRNA biogenesis factors CG2183 (GASZ) and Deadlock. Our data uncover a previously unanticipated set of factors preferentially required for repression of different transposon types.

PMID:
23665227
PMCID:
PMC3724427
DOI:
10.1016/j.molcel.2013.04.007
[Indexed for MEDLINE]
Free PMC Article

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