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Bioconjug Chem. 2013 Jun 19;24(6):960-7. doi: 10.1021/bc300677n. Epub 2013 May 21.

Peptide-based vector of VEGF plasmid for efficient gene delivery in vitro and vessel formation in vivo.

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1
Key Laboratory of Biomedical Polymers of Ministry of Education & Department of Chemistry, Wuhan University, Wuhan 430072, P R China.

Abstract

Critical limb ischemia is regarded as a potentially lethal disease, and the treatment effects of existing therapies are limited. Here, in order to develop a potential approach to improve the therapy effects, we designed a peptide of TAT-PKKKRKV as the vector for VEGF165 plasmid to facilitate in vivo angiogenesis. In in vitro studies, TAT-PKKKRKV with low cytotoxicity exhibited efficient transfection ability either with or without serum. Additionally, application of TAT-PKKKRKV/VEGF165 complexes in hindlimb ischemia rats obviously promoted the expression of VEGF protein, which further enhanced effective angiogenesis. The results indicated that TAT-PKKKRKV is an efficient gene vector with low toxicity both in vitro and in vivo, which has great potential for clinical gene therapy.

PMID:
23663059
DOI:
10.1021/bc300677n
[Indexed for MEDLINE]
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