Format

Send to

Choose Destination
See comment in PubMed Commons below
Semin Cell Dev Biol. 2013 Aug-Sep;24(8-9):661-7. doi: 10.1016/j.semcdb.2013.05.001. Epub 2013 May 7.

Quantitative imaging of subcellular metabolism with stable isotopes and multi-isotope imaging mass spectrometry.

Author information

1
Department of Medicine, Brigham and Women's Hospital and Harvard Medical School, 75 Francis Street, Boston, MA 02115, United States; Division of Genetics, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, United States; Division of Cardiovascular Medicine, Brigham and Women's Hospital, 75 Francis Street, Boston, MA 02115, United States.

Abstract

Multi-isotope imaging mass spectrometry (MIMS) is the quantitative imaging of stable isotope labels in cells with a new type of secondary ion mass spectrometer (NanoSIMS). The power of the methodology is attributable to (i) the immense advantage of using non-toxic stable isotope labels, (ii) high resolution imaging that approaches the resolution of usual transmission electron microscopy and (iii) the precise quantification of label down to 1 part-per-million and spanning several orders of magnitude. Here we review the basic elements of MIMS and describe new applications of MIMS to the quantitative study of metabolic processes including protein and nucleic acid synthesis in model organisms ranging from microbes to humans.

KEYWORDS:

Cell division; Human; Keywords: Stable isotope; Metabolism; Quantitative imaging; Stem cell

PMID:
23660233
PMCID:
PMC3985169
DOI:
10.1016/j.semcdb.2013.05.001
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center