(A) Scatter plots of FAM and Cy5 drop intensities for a control sample (left) and picoinjected sample (right). The gating thresholds used to label a drop as positive or negative for Taqman signal are demarcated by the lines, and divide the scatter plot into quadrants, double negative drops (–,–), FAM positive (–,+), Cy5 positive (+,–), positive for both FAM and Cy5 (+,+). Numbers of drops in each quadrant are indicated. (B) The bar graph shows the average Taqman positive drop count with picoinjection relative to the normalized count for CD44 and EpCAM Taqman assays for control populations. The control detection rate value is defined as 1 for each replicate. The data represent the average of four independent experimental replicates.