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Mol Plant. 2013 Sep;6(5):1661-72. doi: 10.1093/mp/sst071. Epub 2013 May 8.

Adenine phosphoribosyl transferase 1 is a key enzyme catalyzing cytokinin conversion from nucleobases to nucleotides in Arabidopsis.

Author information

1
State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking-Tsinghua Center for Life Sciences, Peking University, Life Sciences Building, 5 Yi He Yuan Road, Haidian, Beijing 100871, China.

Abstract

In plants, the cytokinin metabolic processes, including cytokinin biosynthesis, interconversion, inactivation, and degradation, play critical roles in the regulation of cytokinin homeostasis and plant development. Purine metabolic enzymes have been implied to catalyze the cytokinin interconversion in previous works. In this study, we report that Adenine Phosphoribosyl Transferase 1 (APT1) is the causal gene of the high-dose cytokinin-resistant mutants. APT1 catalyzes the cytokinin conversion from free bases to nucleotides, and is functionally predominant among the five members of the Arabidopsis Adenine Phosphoribosyl Transferase family. Loss of APT1 activity in plants leads to excess accumulation of cytokinin bases, thus evoking myriad cytokinin-regulated responses, such as delayed leaf senescence, anthocyanin accumulation, and downstream gene expression. Thus, our study defines APT1 as a key metabolic enzyme participating in the cytokinin inactivation by phosphoribosylation.

KEYWORDS:

APT1; Arabidopsis thaliana.; cytokinin; phosphoribosylation; plant hormone

PMID:
23658065
DOI:
10.1093/mp/sst071
[Indexed for MEDLINE]
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