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Zhonghua Wei Zhong Bing Ji Jiu Yi Xue. 2013 Mar;25(3):164-6. doi: 10.3760/cma.j.issn.2095-4352.2013.03.011.

[Down-regulation of expression of α- smooth muscle actin in airway smooth muscle cells by allicin and its mechanisms].

[Article in Chinese]

Author information

1
Department of Pulmonology, the First Hospital Affiliated to General Hospital of PLA, Beijing, China. yixueqikanw@126.com

Abstract

OBJECTIVE:

To investigate the effect of allicin on the expression of α-smooth muscle actin (α-SMA) in airway smooth muscle cells (ASMC), and to evaluate the mechanism of allicin on inhibition of airway remodeling.

METHODS:

The human ASMCs were treated for 48 hours with 0, 2.5, 5.0, and 10.0 μmol/L allicin, respectively, and the total RNA and protein of the cells were collected. The mRNA expression of α-SMA was determined by real-time polymerase chain reaction (PCR) analysis. The protein expression of α-SMA and phosphorylation Smad1 (p-Smad1) were assessed by Western blotting analysis.

RESULTS:

After the treatment with allicin for 48 hours in a dose of 0, 2.5, 5.0, and 10.0 μmol/L respectively, the mRNA expression of α-SMA was down-regulated (0.543±0.121, 0.354±0.072, 0.223±0.058, and 0.191±0.034, respectively), with statistically significant difference among groups (all P<0.05), and the protein expression of α-SMA and p-Smad1 was also gradually down-regulated in a dose-dependent manner [α-SMA protein (grad value ratio): 0.96±0.02, 0.72±0.16, 0.54±0.11, and 0.31±0.14, respectively; p-Smad1 protein (grad value ratio): 0.94±0.03, 0.76±0.13, 0.62±0.11, and 0.43±0.12, respectively), with statistically significant difference among groups (all P<0.05).

CONCLUSION:

Allicin depresses the mRNA and protein expression of α-SMA, and inhibits p-Smad1 in a dose-dependent manner, thus interrupts the transforming growth factor-β and Rho kinase signal pathway.

[Indexed for MEDLINE]
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