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Nat Commun. 2013;4:1781. doi: 10.1038/ncomms2807.

Ribosomal protein S1 functions as a termination factor in RNA synthesis by Qβ phage replicase.

Author information

1
Institute of Protein Research of the Russian Academy of Sciences, Pushchino, Moscow 142290, Russia.

Abstract

S1 is the largest ribosomal protein, and is vitally important for the cell. S1 is also a subunit of Qβ replicase, the RNA-directed RNA polymerase of bacteriophage Qβ. In both protein and RNA syntheses, S1 is commonly believed to bind to a template RNA at the initiation step, and not to be involved in later events. Here, we show that in Qβ replicase-mediated RNA synthesis, S1 functions at the termination step by promoting release of the product strand in a single-stranded form. This function is fulfilled by the N-terminal fragment comprising the first two S1 domains. The results suggest that S1 might also have a role other than mRNA binding in the ribosome.

PMID:
23653193
DOI:
10.1038/ncomms2807
[Indexed for MEDLINE]

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