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Cell Physiol Biochem. 2013;31(4-5):649-58. doi: 10.1159/000350084. Epub 2013 May 6.

Expression of aquaporins in human embryos and potential role of AQP3 and AQP7 in preimplantation mouse embryo development.

Author information

1
Key Laboratory of Reproductive Genetics, Zhejiang University, Ministry of Education, Hangzhou, China.

Abstract

BACKGROUND/AIMS:

Water channels, also named aquaporins (AQPs), play crucial roles in cellular water homeostasis.

METHODS:

RT-PCR indicated the mRNA expression of AQPs 1-5, 7, 9, and 11-12, but not AQPs 0, 6, 8, and 10 in the 2∼8-cell stage human embryos. AQP3 and AQP7 were further analyzed for their mRNA expression and protein expression in the oocyte, zygote, 2-cell embryo, 4-cell embryo, 8-cell embryo, morula, and blastocyst from both human and mouse using RT-PCR and immunofluorescence, respectively.

RESULTS:

AQP3 and AQP7 were detected in all these stages. Knockdown of either AQP3 or AQP7 by targeted siRNA injection into 2-cell mouse embryos significantly inhibited preimplantation embryo development. However, knockdown of AQP3 in JAr spheroid did not affect its attachment to Ishikawa cells.

CONCLUSION:

These data demonstrate that multiple aquaporins are expressed in the early stage human embryos and that AQP3 and AQP7 may play a role in preimplantation mouse embryo development.

PMID:
23652731
DOI:
10.1159/000350084
[Indexed for MEDLINE]
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