Format

Send to

Choose Destination
See comment in PubMed Commons below
J Antimicrob Chemother. 2013 Sep;68(9):2083-91. doi: 10.1093/jac/dkt157. Epub 2013 May 6.

Analysis of the tolerance of pathogenic enterococci and Staphylococcus aureus to cell wall active antibiotics.

Author information

1
Université de Caen Basse-Normandie, EA4655 U2RM-Stress and Virulence, F-14032 Caen, France. axel.hartke@unicaen.fr

Abstract

OBJECTIVES:

Tolerance refers to the phenomenon that bacteria do not significantly die when exposed to bactericidal antibiotics. Enterococci are known for their high tolerance to these drugs, but the molecular reasons why they resist killing are not understood. In a previous study we showed that the superoxide dismutase (SOD) is implicated in this tolerance. This conclusion was based on the results obtained with one particular strain of Enterococcus faecalis and therefore the objective of the present communication was to analyse whether dependence of tolerance on active SOD is a general phenomenon for enterococci and another Gram-positive pathogen, Staphylococcus aureus.

METHODS:

Mutants deficient in SOD activity were constructed in pathogenic enterococci. The wild-type sodA gene was cloned into an expression vector and transformed into SOD-deficient strains for complementation with varying levels of SOD activity. Previously constructed SOD-deficient strains of S. aureus were also included in this study. Tolerance to vancomycin and penicillin was then tested.

RESULTS:

We demonstrated that the dependence on SOD of tolerance to vancomycin and penicillin is a common trait of antibiotic-susceptible pathogenic enterococci. By varying the levels of expression we could also show that tolerance to vancomycin is directly correlated to SOD activity. Interestingly, deletion of the sodA gene in a non-tolerant Enterococcus faecium strain did not further sensitize the mutant to bactericidal antibiotics. Finally, we showed that the SOD enzymes of S. aureus are also implicated in tolerance to vancomycin.

CONCLUSION:

High tolerance of enterococci to cell wall active antibiotics can be reversed by SOD deficiency.

KEYWORDS:

bactericidal antibiotics; oxidative stress; superoxide dismutases

PMID:
23649229
DOI:
10.1093/jac/dkt157
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Silverchair Information Systems
    Loading ...
    Support Center