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Virology. 2013 Jul 20;442(1):3-11. doi: 10.1016/j.virol.2013.03.030. Epub 2013 May 4.

Mapping the telomere integrated genome of human herpesvirus 6A and 6B.

Author information

1
Department of Molecular Medicine, University of South Florida College of Medicine, Tampa, FL 33612, United States.

Abstract

Human herpesvirus 6B (HHV-6B) is the causative agent of roseola infantum. HHV-6A and 6B can reactivate in immunosuppressed individuals and are linked with severe inflammatory response, organ rejection and central nervous system diseases. About 0.85% of the US and UK population carries an integrated HHV-6 genome in all nucleated cells through germline transmission. We have previously reported that the HHV-6A genome integrated in telomeres of patients suffering from neurological dysfunction and also in telomeres of tissue culture cells. We now report that HHV-6B also integrates in telomeres during latency. Detailed mapping of the integrated viral genomes demonstrates that a single HHV-6 genome integrates and telomere repeats join the left end of the integrated viral genome. When HEK-293 cells carrying integrated HHV-6A were exposed to the histone deacetylase inhibitor Trichostatin A, circularization and/or formation of concatamers were detected and this assay could be used to distinguish between lytic replication and latency.

PMID:
23648233
PMCID:
PMC3696530
DOI:
10.1016/j.virol.2013.03.030
[Indexed for MEDLINE]
Free PMC Article

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