Phage display antibodies for diagnostic applications

Nur Hidayah Hairul Bahara; Gee Jun Tye; Yee Siew Choong; Eugene Boon Beng Ong; Asma Ismail; Theam Soon Lim

doi:10.1016/j.biologicals.2013.04.001

Phage display antibodies for diagnostic applications

Biologicals. 2013 Jul;41(4):209-16. doi: 10.1016/j.biologicals.2013.04.001. Epub 2013 May 3.

Authors

Nur Hidayah Hairul Bahara¹, Gee Jun Tye, Yee Siew Choong, Eugene Boon Beng Ong, Asma Ismail, Theam Soon Lim

Affiliation

¹ Institute for Research in Molecular Medicine, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia.

PMID: 23647952
DOI: 10.1016/j.biologicals.2013.04.001

Abstract

With major developments in molecular biology, numerous display technologies have been successfully introduced for recombinant antibody production. Even so, phage display still remains the gold standard for recombinant antibody production. Its success is mainly attributed to the robust nature of phage particles allowing for automation and adaptation to modifications. The generation of monospecific binders provides a vital tool for diagnostics at a lower cost and higher efficiency. The flexibility to modify recombinant antibodies allows great applicability to various platforms for use. This review presents phage display technology, application and modifications of recombinant antibodies for diagnostics.

Keywords: Antibodies; Diagnostics; Phage display; scFv.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Animals
Gene Library*
Humans
Recombinant Proteins / biosynthesis
Recombinant Proteins / genetics
Single-Chain Antibodies* / biosynthesis
Single-Chain Antibodies* / genetics

Substances

Recombinant Proteins
Single-Chain Antibodies