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ACS Nano. 2013 Jun 25;7(6):5207-14. doi: 10.1021/nn4009388. Epub 2013 May 9.

Fast and efficient molecule detection in localization-based super-resolution microscopy by parallel adaptive histogram equalization.

Author information

1
Institute of Applied Physics and Center for Functional Nanostructures (CFN), Karlsruhe Institute of Technology (KIT), Wolfgang-Gaede-Strasse 1, 76131 Karlsruhe, Germany.

Abstract

In localization-based super-resolution microscopy, individual fluorescent markers are stochastically photoactivated and subsequently localized within a series of camera frames, yielding a final image with a resolution far beyond the diffraction limit. Yet, before localization can be performed, the subregions within the frames where the individual molecules are present have to be identified-oftentimes in the presence of high background. In this work, we address the importance of reliable molecule identification for the quality of the final reconstructed super-resolution image. We present a fast and robust algorithm (a-livePALM) that vastly improves the molecule detection efficiency while minimizing false assignments that can lead to image artifacts.

PMID:
23647371
DOI:
10.1021/nn4009388

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