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Anticancer Res. 2013 May;33(5):1881-5.

Changes in CO2 concentration increase the invasive ability of colon cancer cells.

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1
First Department of Surgery, University of Fukui, 23-3, Eiheiji-cho, Yoshida-gun, Fukui, Japan.

Abstract

BACKGROUND/AIM:

We studied the effects of CO(2) concentration changes on the invasive ability of colon cancer cells.

MATERIALS AND METHODS:

Colon cancer cell lines and human samples derived from a peritoneal metastasis were incubated in a hypercapnic environment, followed by incubation in 5% CO(2). The invasive ability of colon cancer cells incubated with CO(2) were analyzed using an invasion assay system.

RESULTS:

In comparison with the colon cancer cell lines incubated in 5% CO(2) only, the invasive ability of cells increased in all the colon cancer cell lines subjected to incubation in 20% CO(2) followed by incubation in 5% CO(2), with a concomitant increase in the mRNA expressions of matrix metalloproteinase-2 (MMP2) and MMP9. The invasive capability of peritoneal metastatic cells in the human-derived specimen also increased on CO(2) concentration changes.

CONCLUSION:

CO(2) concentration changes enhanced the invasive capacity of colon cancer cells.

KEYWORDS:

CO2; Colon cancer; insufflation; invasion; laparoscopy

PMID:
23645734
[Indexed for MEDLINE]
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