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RNA. 2013 Jul;19(7):889-95. doi: 10.1261/rna.038232.113. Epub 2013 May 3.

RNA localization in Xenopus oocytes uses a core group of trans-acting factors irrespective of destination.

Author information

1
Department of Chemistry and Biochemistry, University of Notre Dame, Notre Dame, Indiana 46556, USA.

Abstract

The 3' untranslated region of mRNA encoding PHAX, a phosphoprotein required for nuclear export of U-type snRNAs, contains cis-acting sequence motifs E2 and VM1 that are required for localization of RNAs to the vegetal hemisphere of Xenopus oocytes. However, we have found that PHAX mRNA is transported to the opposite, animal, hemisphere. A set of proteins that cross-link to the localization elements of vegetally localized RNAs are also cross-linked to PHAX and An1 mRNAs, demonstrating that the composition of RNP complexes that form on these localization elements is highly conserved irrespective of the final destination of the RNA. The ability of RNAs to bind this core group of proteins is correlated with localization activity. Staufen1, which binds to Vg1 and VegT mRNAs, is not associated with RNAs localized to the animal hemisphere and may determine, at least in part, the direction of RNA movement in Xenopus oocytes.

KEYWORDS:

PHAX; RNA localization; Staufen; Vg1 mRNA; Xenopus oocyte

PMID:
23645708
PMCID:
PMC3683923
DOI:
10.1261/rna.038232.113
[Indexed for MEDLINE]
Free PMC Article

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