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Nat Struct Mol Biol. 2013 Jun;20(6):763-5. doi: 10.1038/nsmb.2574. Epub 2013 May 5.

CENP-A confers a reduction in height on octameric nucleosomes.

Author information

1
Wellcome Trust Centre for Cell Biology and Institute of Cell Biology, School of Biological Sciences, University of Edinburgh, Edinburgh, Scotland, UK.

Abstract

Nucleosomes with histone H3 replaced by CENP-A direct kinetochore assembly. CENP-A nucleosomes from human and Drosophila have been reported to have reduced heights as compared to canonical octameric H3 nucleosomes, thus suggesting a unique tetrameric hemisomal composition. We demonstrate that octameric CENP-A nucleosomes assembled in vitro exhibit reduced heights, indicating that they are physically distinct from H3 nucleosomes and negating the need to invoke the presence of hemisomes.

PMID:
23644598
PMCID:
PMC3676451
DOI:
10.1038/nsmb.2574
[Indexed for MEDLINE]
Free PMC Article

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