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Appl Biochem Biotechnol. 2013 Jul;170(5):1119-26. doi: 10.1007/s12010-013-0260-9. Epub 2013 May 3.

Three-phase partitioning as a rapid and easy method for the purification and recovery of catalase from sweet potato tubers (Solanum tuberosum).

Author information

1
Section of Biochemistry, Department of Chemistry, Faculty of Arts and Sciences, Kocaeli University, Umuttepe Central Campus, 41380 ─░zmit, Kocaeli, Turkey. yonca.avciduman@gmail.com

Abstract

Three-phase partitioning (TPP) was used to purify and recover catalase from potato crude extract. The method consists of ammonium sulfate saturation, t-butanol addition, and adjustment of pH, respectively. The best catalase recovery (262 %) and 14.1-fold purification were seen in the interfacial phase in the presence of 40 % (w/v) ammonium sulfate saturation with 1.0:1.0 crude extract/t-butanol ratio (v/v) at pH 7 in a single step. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of the enzyme showed comparatively purification and protein molecular weight was nearly found to be 56 kDa. This study shows that TPP is a simple, economical, and quick method for the recovering of catalase and can be used for the purification process.

PMID:
23640263
DOI:
10.1007/s12010-013-0260-9
[Indexed for MEDLINE]

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