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Proc Natl Acad Sci U S A. 2013 May 14;110(20):8272-7. doi: 10.1073/pnas.1220398110. Epub 2013 Apr 29.

Dynamics of internal pore opening in K(V) channels probed by a fluorescent unnatural amino acid.

Author information

1
Groupe d'étude des protéines membranaires (GÉPROM) and Department of Physics, Université de Montréal, Montreal, QC, Canada H3C 3J7.

Abstract

Atomic-scale models on the gating mechanism of voltage-gated potassium channels (Kv) are based on linear interpolations between static structures of their initial and final state derived from crystallography and molecular dynamics simulations, and, thus, lack dynamic structural information. The lack of information on dynamics and intermediate states makes it difficult to associate the structural with the dynamic functional data obtained with electrophysiology. Although voltage-clamp fluorometry fills this gap, it is limited to sites extracellularly accessible, when the key region for gating is located at the cytosolic side of the channels. Here, we solved this problem by performing voltage-clamp fluorometry with a fluorescent unnatural amino acid. By using an orthogonal tRNA-synthetase pair, the fluorescent unnatural amino acid was incorporated in the Shaker voltage-gated potassium channel at key regions that were previously inaccessible. Thus, we defined which parts act independently and which parts act cooperatively and found pore opening to occur in two sequential transitions.

KEYWORDS:

Anap; two-color VCF

PMID:
23630265
PMCID:
PMC3657800
DOI:
10.1073/pnas.1220398110
[Indexed for MEDLINE]
Free PMC Article

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