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Regen Med. 2013 May;8(3):295-308. doi: 10.2217/rme.13.25.

Transient overexpression of Pparγ2 and C/ebpα in mesenchymal stem cells induces brown adipose tissue formation.

Author information

1
Skeletal Biotech Laboratory, Hebrew University-Hadassah, Faculty of Dental Medicine, Jerusalem, Israel.

Abstract

BACKGROUND:

Brown adipose tissue plays a pivotal role in mammal metabolism and thermogenesis. It has a great therapeutic potential in several metabolic disorders such as obesity and diabetes. Mesenchymal stem cells (MSCs) are suitable candidates for brown adipose tissue formation de novo. Pparγ2 and C/ebpα are nucleic receptors known to mediate adipogenic differentiation. We hypothesized that overexpression of the Pparγ2 and C/ebpα genes in MSCs would lead to the formation of adipose tissue.

MATERIALS & METHODS:

MSCs bearing the Luc reporter gene were transfected to overexpress Pparγ2 and C/ebpα. Differentiation of nucleofected cells was evaluated in vitro and in vivo following ectopic implantation of the cells in C3H/HeN mice.

RESULTS:

After implantation, the engineered cells survived for 5 weeks and brown adipose-like tissue was observed in histological samples. Immunostaining and bioluminescent imaging showed new adipocytes expressing Luc and the brown adipose tissue marker, UCP1, in vitro and in vivo.

CONCLUSION:

We show that gene delivery of transcription factors into MSCs generates brown adipose tissue in vitro and in vivo.

PMID:
23627824
DOI:
10.2217/rme.13.25
[Indexed for MEDLINE]
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