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[Development and evaluation of a quantitative double antibodies sandwich ELISA assay for rIFN-alpha1b].

[Article in Chinese]

Author information

1
Daxing Industrial Development Zone, Beijing, China. mywu5@sohu.com

Abstract

OBJECTIVE:

To develop a double antibody sandwich ELISA assay for quantitative determination of recombinant human interferon alpha1b.

METHODS:

Mouse monoclonal antibodies with different binding site on rIFN-alpha1b were screened to select optimized candidates as coating and HRP-labeled index antibodies respectively. And a double antibodies sandwich ELISA was assembled; the reliable lower detection limit, specificity, accuracy and reproducibility were evaluated and validated.

RESULTS:

The quantitative sandwich ELISA had a reliable lower detection limit of 10 ng/ml, with a liner detection range 10-100 ng/ml (R2 = 0.992), variation coefficient inter-plates is less than 10%.

CONCLUSION:

The developed sandwich ELISA was a sensitive and specific, accuracy and reproducibility method for quantitative determination of recombinant human interferon alpha1b in final product.

PMID:
23627039
[Indexed for MEDLINE]
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