Format

Send to

Choose Destination
See comment in PubMed Commons below

[Detection of hepatitis A virus RNA with an improved loop-mediated isothermal amplification assay].

[Article in Chinese]

Author information

1
National Institute of Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 102206, China.

Abstract

OBJECTIVE:

To establish a novel improved loop-mediated isothermal amplification (LAMP) technique to detect hepatitis A virus (HAV).

METHODS:

A novel improved LAMP assay based on the addition of an acceleration primer was developed for hepatitis A virus nucleotide acid detection.

RESULTS:

Precision and reproducibility analysis proved its high stability and reliability. Comparison between the improved and conventional LAMP assays revealed that the former was more sensitive with a detection limit of 5 TCID50/ml. The novel detection method displayed 100% consistency with the TaqMan real-time PCR assay when applied to clinical specimens collected from patients with confirmed acute HAV infection.

CONCLUSION:

This novel technique is widely applicable as a simple diagnostic tool in the clinical field as well as for the surveillance and investigation of the infectious disease in developing countries where HAV is endemic.

PMID:
23627037
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Loading ...
    Support Center