Abstract
We screened for a Rab39-specific effector by performing a yeast two-hybrid assay with GTP-locked Rab39A/B as the bait and identified UACA (uveal autoantigen with coiled-coil domains and ankyrin repeats) as a specific Rab39A/B-binding protein. Deletion analysis revealed that a C-terminal coiled-coil domain of UACA functions as a GTP-dependent Rab39-binding domain. shRNA-mediated knockdown of endogenous Rab39A or UACA in mouse neuroblastoma Neuro2A cells resulted in a change in retinoic acid-induced neurite morphology from a multipolar morphology to a bipolar morphology. Taken together, these findings indicate that UACA functions as a Rab39A effector in the retinoic acid-induced differentiation of Neuro2A cells.
Copyright © 2013 Elsevier Inc. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Animals
-
COS Cells
-
Cell Differentiation / drug effects
-
Cell Line, Tumor
-
Chlorocebus aethiops
-
Green Fluorescent Proteins / genetics
-
Green Fluorescent Proteins / metabolism
-
Immunoblotting
-
Membrane Proteins / genetics
-
Membrane Proteins / metabolism*
-
Mice
-
Microscopy, Confocal
-
Microscopy, Fluorescence
-
Monomeric GTP-Binding Proteins / genetics
-
Monomeric GTP-Binding Proteins / metabolism
-
Neurites / drug effects*
-
Neurites / physiology
-
Neuroblastoma / genetics
-
Neuroblastoma / metabolism
-
Neuroblastoma / pathology
-
Protein Binding
-
RNA Interference
-
Tretinoin / pharmacology*
-
Two-Hybrid System Techniques
-
rab GTP-Binding Proteins / genetics
-
rab GTP-Binding Proteins / metabolism*
Substances
-
Membrane Proteins
-
Uaca protein, mouse
-
Green Fluorescent Proteins
-
Tretinoin
-
Rab39A protein, mouse
-
Monomeric GTP-Binding Proteins
-
rab GTP-Binding Proteins