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Cell. 2013 Apr 25;153(3):640-53. doi: 10.1016/j.cell.2013.03.044.

14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins.

Author information

1
Randall Division of Cell and Molecular Biophysics, New Hunt's House, King's College London, London, UK.

Erratum in

  • Cell. 2013 May 23;153(5):1164.

Abstract

Signaling through G proteins normally involves conformational switching between GTP- and GDP-bound states. Several Rho GTPases are also regulated by RhoGDI binding and sequestering in the cytosol. Rnd proteins are atypical constitutively GTP-bound Rho proteins, whose regulation remains elusive. Here, we report a high-affinity 14-3-3-binding site at the C terminus of Rnd3 consisting of both the Cys241-farnesyl moiety and a Rho-associated coiled coil containing protein kinase (ROCK)-dependent Ser240 phosphorylation site. 14-3-3 binding to Rnd3 also involves phosphorylation of Ser218 by ROCK and/or Ser210 by protein kinase C (PKC). The crystal structure of a phosphorylated, farnesylated Rnd3 peptide with 14-3-3 reveals a hydrophobic groove in 14-3-3 proteins accommodating the farnesyl moiety. Functionally, 14-3-3 inhibits Rnd3-induced cell rounding by translocating it from the plasma membrane to the cytosol. Rnd1, Rnd2, and geranylgeranylated Rap1A interact similarly with 14-3-3. In contrast to the canonical GTP/GDP switch that regulates most Ras superfamily members, our results reveal an unprecedented mechanism for G protein inhibition by 14-3-3 proteins.

Comment in

PMID:
23622247
PMCID:
PMC3690454
DOI:
10.1016/j.cell.2013.03.044
[Indexed for MEDLINE]
Free PMC Article

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