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Asian Pac J Trop Biomed. 2013 Apr;3(4):297-302. doi: 10.1016/S2221-1691(13)60066-0.

Combinational effects of hexane insoluble fraction of Ficus septica Burm. F. and doxorubicin chemotherapy on T47D breast cancer cells.

Author information

1
Cancer Chemoprevention Research Center, Faculty of Pharmacy, Gadjah Mada University, Jogjakarta 55281, Indonesia.

Abstract

OBJECTIVE:

To evaluate the effects of n-hexane insoluble fraction (HIF) of Ficus septica leaves in combination with doxorubicin on cytotoxicity, cell cycle and apoptosis induction of breast cancer T47D cell lines.

METHODS:

The in vitro drugs-stimulated cytotoxic effects were determined using MTT assay. Analysis of cell cycle distribution was performed using flowcytometer and the data was analyzed using ModFit LT 3.0 program. Apoptosis assay was carried out by double staining method using ethydium bromide-acridin orange. The expression of cleaved-poly (ADP-ribose) polymerase (PARP) on T47D cell lines was identified using immunocytochemistry.

RESULTS:

The combination exhibited higher inhibitory effect on cell growth than the single treatment of doxorubicin in T47D cells. In addition, combination of doxorubicin and HIF increased the incidence of cells undergoing apoptosis. HIF could improve doxorubicin cytotoxic effect by changing the accumulation of cell cycle phase from G2/M to G1 phase. The combination also exhibited upregulation of cleaved-PARP in T47D cells.

CONCLUSIONS:

Based on this results, HIF is potential to be developed as co-chemotherapeutic agent for breast cancer by inducing apoptosis and cell cycle arrest. However, the molecular mechanism need to be explored further.

KEYWORDS:

Cancer; Cytotoxic; Ficus septica Burm. F.; T47D cells

PMID:
23620854
PMCID:
PMC3634927
DOI:
10.1016/S2221-1691(13)60066-0
[Indexed for MEDLINE]
Free PMC Article

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