Format

Send to

Choose Destination
Talanta. 2013 May 15;109:173-6. doi: 10.1016/j.talanta.2013.02.001. Epub 2013 Feb 8.

Label-free detection of single-stranded DNA binding protein based on a cantilever array.

Author information

1
Department of Chemistry, Nanchang University, Nanchang 330031, PR China.

Abstract

We report a simple and sensitive method for label-free detection of single-stranded DNA-binding protein (SSBP) based on an array of microfabricated cantilevers. The single-stranded DNA (ssDNA) was immobilized on the surface of the sensing cantilevers to detect SSBP, while the reference cantilevers were modified with 6-mercapto-1-hexanol to detect any unwanted cantilever deflection. The differential deflection signals that reveal specific SSBP-ssDNA binding have been found to depend on the SSBP concentration. Using the cantilever array sensor we can detect SSBP in the concentration range from 0.01 to 7 μg mL(-1). Other proteins, such as thrombin or bovine serum albumin induced no significant deflection of the cantilevers. Our results show the potential for the application of cantilever array sensor system as a powerful tool to detect proteins with high sensitivity and specificity.

PMID:
23618156
DOI:
10.1016/j.talanta.2013.02.001
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center