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Proteomics. 2013 Oct;13(18-19):2761-5. doi: 10.1002/pmic.201300010. Epub 2013 Jun 18.

Surfaceomics and surface-enhanced Raman spectroscopy of environmental microbes: matching cofactors with redox-active surface proteins.

Author information

1
Department of Plant and Microbial Biology, University of California, Berkeley, CA, USA.

Abstract

Trypsin shaving is a targeted proteomic method for identifying cell-surface exposed proteins on bacterial cells. For the identification of redox-active cell-surface proteins, trypsin-shaving datasets can be matched with surface-enhanced Raman spectra of intact cells to identify the cofactors associated with the cell-surface proteins. Together, these approaches could help resolve questions about the presence of cell-surface electron transport components in environmental microorganisms, especially microbes that oxidize and reduce metals and metalloids as electron donors and acceptors.

KEYWORDS:

Microbiology; SERRS; SERS; Surfaceome; Trypsin shaving

PMID:
23616463
DOI:
10.1002/pmic.201300010
[Indexed for MEDLINE]

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