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Proc Natl Acad Sci U S A. 2013 May 7;110(19):7748-53. doi: 10.1073/pnas.1304049110. Epub 2013 Apr 22.

Interdomain lateral gene transfer of an essential ferrochelatase gene in human parasitic nematodes.

Author information

1
Division of Molecular Parasitology, New England Biolabs, Inc, Ipswich, MA 01938, USA.

Abstract

Lateral gene transfer events between bacteria and animals highlight an avenue for evolutionary genomic loss/gain of function. Herein, we report functional lateral gene transfer in animal parasitic nematodes. Members of the Nematoda are heme auxotrophs, lacking the ability to synthesize heme; however, the human filarial parasite Brugia malayi has acquired a bacterial gene encoding ferrochelatase (BmFeCH), the terminal step in heme biosynthesis. BmFeCH, encoded by a 9-exon gene, is a mitochondrial-targeted, functional ferrochelatase based on enzyme assays, complementation, and inhibitor studies. Homologs have been identified in several filariae and a nonfilarial nematode. RNAi and ex vivo inhibitor experiments indicate that BmFeCH is essential for viability, validating it as a potential target for filariasis control.

PMID:
23610429
PMCID:
PMC3651471
DOI:
10.1073/pnas.1304049110
[Indexed for MEDLINE]
Free PMC Article

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