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J Plant Physiol. 2013 Sep 1;170(13):1212-5. doi: 10.1016/j.jplph.2013.03.011. Epub 2013 Apr 19.

Endogenous abscisic acid is involved in methyl jasmonate-induced reactive oxygen species and nitric oxide production but not in cytosolic alkalization in Arabidopsis guard cells.

Author information

1
Graduate School of Natural Science and Technology, Okayama University, 1-1-1 Tsushima-Naka, Okayama 700-8530, Japan.

Abstract

We recently demonstrated that endogenous abscisic acid (ABA) is involved in methyl jasmonate (MeJA)-induced stomatal closure in Arabidopsis thaliana. In this study, we investigated whether endogenous ABA is involved in MeJA-induced reactive oxygen species (ROS) and nitric oxide (NO) production and cytosolic alkalization in guard cells using an ABA-deficient Arabidopsis mutant, aba2-2, and an inhibitor of ABA biosynthesis, fluridon (FLU). The aba2-2 mutation impaired MeJA-induced ROS and NO production. FLU inhibited MeJA-induced ROS production in wild-type guard cells. Pretreatment with 0.1 μM ABA, which does not induce stomatal closure in the wild type, complemented the insensitivity to MeJA of the aba2-2 mutant. However, MeJA induced cytosolic alkalization in both wild-type and aba2-2 guard cells. These results suggest that endogenous ABA is involved in MeJA-induced ROS and NO production but not in MeJA-induced cytosolic alkalization in Arabidopsis guard cells.

KEYWORDS:

2′,7′-bis(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester; 2′,7′-dichlorodihydrofluorescein diacetate; 4,5-diaminofluorescein-2 diacetate; ABA; Arabidopsis thaliana; BCECF-AM; Cytosolic alkalization; DAF-2DA; FLU; H(2)DCF-DA; MeJA; Methyl jasmonate; Nitric oxide; ROS; Reactive oxygen species; [Ca(2+)](cyt); abscisic acid; cytosolic free calcium concentration; cytosolic pH; fluridon; methyl jasmonate; pH(cyt); reactive oxygen species

PMID:
23608742
DOI:
10.1016/j.jplph.2013.03.011
[Indexed for MEDLINE]

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