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Genetics. 2013 Jun;194(2):447-57. doi: 10.1534/genetics.113.150789. Epub 2013 Apr 22.

AKAP9 is essential for spermatogenesis and sertoli cell maturation in mice.

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1
Department of Biology, Middlebury College, Middlebury, VT 05753, USA.

Abstract

Mammalian male fertility relies on complex inter- and intracellular signaling during spermatogenesis. Here we describe three alleles of the widely expressed A-kinase anchoring protein 9 (Akap9) gene, all of which cause gametogenic failure and infertility in the absence of marked somatic phenotypes. Akap9 disruption does not affect spindle nucleation or progression of prophase I of meiosis but does inhibit maturation of Sertoli cells, which continue to express the immaturity markers anti-Mullerian hormone and thyroid hormone receptor alpha in adults and fail to express the maturation marker p27(Kip1). Furthermore, gap and tight junctions essential for blood-testis barrier (BTB) organization are disrupted. Connexin43 (Cx43) and zona occludens-1 are improperly localized in Akap9 mutant testes, and Cx43 fails to compartmentalize germ cells near the BTB. These results identify and support a novel reproductive tissue-specific role for Akap9 in the coordinated regulation of Sertoli cells in the testis.

KEYWORDS:

Sertoli cells; infertility; mouse; protein kinase A; spermatogenesis

PMID:
23608191
PMCID:
PMC3664854
DOI:
10.1534/genetics.113.150789
[Indexed for MEDLINE]
Free PMC Article

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