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Theranostics. 2013 Mar 13;3(4):239-48. doi: 10.7150/thno.5113. Print 2013.

Multicolor upconversion nanoparticles for protein conjugation.

Author information

1
University of Regensburg, Institute of Analytical Chemistry, Chemo- and Biosensors, 93040 Regensburg, Germany.

Abstract

We describe the preparation of monodisperse, lanthanide-doped hexagonal-phase NaYF4 upconverting luminescent nanoparticles for protein conjugation. Their core was coated with a silica shell which then was modified with a poly(ethylene glycol) spacer and N-hydroxysuccinimide ester groups. The nanoparticles were characterized by transmission electron microscopy, Raman spectroscopy, X-ray diffraction, and dynamic light scattering. The N-hydroxysuccinimide ester functionalization renders them highly reactive towards amine nucleophiles (e.g., proteins). We show that such particles can be conjugated to proteins. The protein-reactive UCLNPs and their conjugates to streptavidin and bovine serum albumin display multicolor emissions upon 980-nm continuous wave laser excitation. Surface plasmon resonance studies were carried out to prove bioconjugation and to compare the affinity of the particles for proteins immobilized on a thin gold film.

KEYWORDS:

Raman spectroscopy; Upconversion luminescence; biosensing; magnetic separation; nanoparticle; silica coating; surface plasmon resonance.

PMID:
23606910
PMCID:
PMC3630524
DOI:
10.7150/thno.5113
[Indexed for MEDLINE]
Free PMC Article
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