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J Tissue Eng Regen Med. 2016 Jun;10(6):466-74. doi: 10.1002/term.1742. Epub 2013 Apr 18.

Ameloblasts serum-free conditioned medium: bone morphogenic protein 4-induced odontogenic differentiation of mouse induced pluripotent stem cells.

Liu L1,2, Liu YF3, Zhang J2,4, Duan YZ4, Jin Y2,5.

Author information

1
Department of Stomatology, General Hospital of Beijing Military Command, Beijing, 100700, People's Republic of China.
2
Research and Development Centre for Tissue Engineering, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, Shaanxi, People's Republic of China.
3
Department of Stomatology, The 464 Hospital of Beijing PLA, Tianjin, 300191, People's Republic of China.
4
Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, Shaanxi, People's Republic of China.
5
Department of Oral Histology and Pathology, School of Stomatology, Fourth Military Medical University, Xi'an, 710032, Shaanxi, People's Republic of China.

Abstract

Induced pluripotent stem (iPS) cells possess the ability of self-renewal and can differentiate into cells of the three germ layers, both in vitro and in vivo. Here we report a new method to efficiently induce differentiation of mouse iPS cells into the odontogenic lineage. Using ameloblasts serum-free conditioned medium (ASF-CM), we successfully generated ameloblast-like cells from mouse iPS cells. Importantly, culturing mouse iPS cells in ASF-CM supplemented with BMP4 (ASF-BMP4) promoted odontogenic differentiation, which was evident by the upregulation of ameloblast-specific as well as odontoblast-specific genes. On the other hand, culturing mouse iPS cells in ASF-CM supplemented with noggin (ASF-noggin), an inhibitor of BMP4, abrogated this effect. These results suggest that mouse iPS cells can be induced by ASF-BMP4 to differentiate into ameloblast-like and odontoblast-like cells. The results of our study raise the possibility of using patient-specific iPS cells for tooth regeneration in the future. Copyright © 2016 John Wiley & Sons, Ltd.

KEYWORDS:

ameloblasts serum-free conditioned medium; bone morphogenic protein 4; microenviroment; mouse induced pluripotent stem cells; odontogenic lineage; tooth regeneration

PMID:
23606575
DOI:
10.1002/term.1742
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