Simultaneous determination of type A-& B-trichothecenes and zearalenone in cereals by High Performance Liquid Chromatography - Tandem Mass Spectrometry

R Schuhmacher; F Berthiller; G Buttinger; R Krska

doi:10.1007/BF02957584

Simultaneous determination of type A-& B-trichothecenes and zearalenone in cereals by High Performance Liquid Chromatography - Tandem Mass Spectrometry

Mycotoxin Res. 2005 Dec;21(4):237-40. doi: 10.1007/BF02957584.

Authors

R Schuhmacher¹, F Berthiller, G Buttinger, R Krska

Affiliation

¹ Christian Doppler Laboratory for Mycotoxin Research, Department for Agrobiotechnology (IFA-Tulln), BOKU-University of Natural Resources and Applied Life Sciences Vienna, Konrad Lorenz Str. 20, 3430, Tulln, Austria, rainer.schuhmacher@boku.ac.at.

PMID: 23605394
DOI: 10.1007/BF02957584

Abstract

A rapid quantitative method for the simultaneous determination of the majorFusarium mycotoxins nivalenol, deoxynivalenol, fusarenon-X, 3-acetyl-deoxynivalenol, 15-acetyl-deoxynivalenol, diacetoxyscirpenol, HT-2 toxin, T-2 toxin and zearalenone in maize and wheat was developed. Raw extracts (acetonitrile/water 84/16) are cleaned-up with MycoSep(®) columns., Chromatographic separation and end determination is carried out by HPLC-APCI-MS/MS.HPLC run times of 10 minutes considerably increases sample throughput and make this method suitable for routine analysis. The use of a triple quadrupole mass spectrometer allows the selective detection of the mycotoxins and their quantification in the low μg/kg-range.