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RNA Biol. 2013 Jun;10(6):991-1001. doi: 10.4161/rna.24644. Epub 2013 Apr 11.

RNA-seq identified a super-long intergenic transcript functioning in adipogenesis.

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Laboratory of Nucleic Acid Technology and Laboratory of State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, School of Pharmaceutical Sciences, Peking University, Beijing, China.


RNA transcripts are generally classified into polyA-plus and polyA-minus subgroups due to the presence or absence of a polyA tail at the 3' end. Even though a number of physiologically and pathologically important polyA-minus RNAs have been recently identified, a systematic analysis of the expression and function of these transcripts in adipogenesis is still elusive. To study the potential function of the polyA-minus RNAs in adipogenesis, a dynamic expressional profiling was performed in the induced differentiation of 3T3-L1 cells. In addition to identifying thousands of novel intergenic transcripts, differentiation-synchronized expression was characterized for many of them. Among these, several large intergenic transcripts were found to be upregulated by more than 19-fold during differentiation. Further study demonstrated a fat tissue-specific expression pattern for these regions and identified an adipogenesis-associated long non-coding RNA. Collectively, these lines of evidence contribute to the characterization of a super-long intergenic transcript functioning in adipogenesis.


adipogenesis; high-throughput sequencing; large intergenic non-coding RNA; obesity; polyA-minus RNA

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