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Plant Signal Behav. 2013 Jun;8(6):e24510. doi: 10.4161/psb.24510. Epub 2013 Apr 9.

MAP kinases phosphorylate rice WRKY45.

Author information

1
Disease Resistant Crops Research Unit; National Institute of Agrobiological Sciences; Tsukuba, Ibaraki, Japan.

Abstract

WRKY45 transcription factor is a central regulator of disease resistance mediated by the salicylic acid (SA) signaling pathway in rice. SA-activated WRKY45 protein induces the accumulation of its own mRNA. However, the mechanism underlying this regulation is still unknown. Here, we report three lines of evidence showing that a mitogen-activated protein kinase (MAPK) cascade is involved in this regulation. An inhibitor of MAPK kinase (MAPKK) suppressed the increase in WRKY45 transcript level in response to SA. Two MAPKs, OsMPK4 and OsMPK6, phosphorylated WRKY45 protein in vitro. The activity of OsMPK6 was rapidly upregulated by SA treatment in rice cells. These results suggest that WRKY45 is regulated by MAPK-dependent phosphorylation in the SA pathway.

KEYWORDS:

MAPK; disease resistance; phosphorylation; proteasome; rice; salicylic acid

PMID:
23603961
PMCID:
PMC3908937
DOI:
10.4161/psb.24510
[Indexed for MEDLINE]
Free PMC Article

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