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Bioorg Med Chem. 2013 Oct 15;21(20):6139-44. doi: 10.1016/j.bmc.2013.03.054. Epub 2013 Apr 2.

Screening for inhibitors of the hepatitis C virus internal ribosome entry site RNA.

Author information

1
Department of Chemistry and Biochemistry, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.

Abstract

The highly conserved internal ribosome entry site (IRES) of hepatitis C virus (HCV) regulates translation of the viral RNA genome and is essential for the expression of HCV proteins in infected host cells. The structured subdomain IIa of the IRES element is the target site of recently discovered benzimidazole inhibitors that selectively block viral translation through capture of an extended conformation of an RNA internal loop. Here, we describe the development of a FRET-based screening assay for similarly acting HCV translation inhibitors. The assay relies on monitoring fluorescence changes that indicate rearrangement of the RNA target conformation upon ligand binding. Screening of a small pilot set of potential RNA binders identified a benzoxazole scaffold as a ligand that bound selectively to IIa IRES target and was confirmed as an inhibitor of in vitro viral translation. The screening approach outlined here provides an efficient method to discover HCV translation inhibitors that may provide leads for the development of novel antiviral therapies directed at the highly conserved IRES RNA.

KEYWORDS:

Benzimidazoles; Benzoxazoles; FRET assay; RNA targeting

PMID:
23602522
PMCID:
PMC3758467
DOI:
10.1016/j.bmc.2013.03.054
[Indexed for MEDLINE]
Free PMC Article

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