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Biomed Pharmacother. 2013 Jun;67(5):381-6. doi: 10.1016/j.biopha.2013.03.013. Epub 2013 Apr 3.

MiR-223/Ect2/p21 signaling regulates osteosarcoma cell cycle progression and proliferation.

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Department of Orthopaedic Surgery, Beijing Shijitan Hospital Affiliated to Capital Medical University, Beijing, PR China.


Osteosarcoma is one of the most common tumors. The mechanisms of formation and development of osteosarcoma have been studied for a long time. Recently, more and more evidence showed that miRNAs play important roles in regulating tumor growth. In this study we found that miRNA-223 was downregulated in both osteosarcoma patients' tumor tissues and osteosarcoma cell lines. Overexpression of miRNA-233 greatly inhibited the proliferation of Saos-2 cells. Cell cycle analysis by flow cytometry showed the arrest of cell cycle progression at the G1 phase. Further mechanistic study indicated that Ect2 was directly targeted by miR-223. Downregulation of Ect2 by miR-223 induces the expression of p21, p27 and the phospharylation of retinoblastoma, which are involved in the G1 block. We concluded that miR-223 functions as a tumor suppresser in osteosarcoma and miR-223/Ect2/p21 signaling is an important pathway that regulates the osteosarcoma cell cycle progression and proliferaion.

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