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Biophys J. 2013 Apr 16;104(8):1805-11. doi: 10.1016/j.bpj.2013.01.063.

Assessment of 0.5 T static field exposure effect on yeast and HEK cells using electrorotation.

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1
Université de Lyon - CNRS UMR5005 Laboratoire Ampère- Université Claude Bernard Lyon 1 - École Centrale de Lyon Ecully. amal.el-gaddar@ec-lyon.fr

Abstract

This study aims to examine the influence of a 0.5 T static magnetic field (SMF) on yeast and human embryonic kidney (HEK) 293 cell using electrorotation (ROT). Following 48 h exposition to the SMF, no difference was noted between ROT spectra of unexposed and exposed yeast cells, which extend previous reports on the absence of SMF effects on yeast. We also compared the ROT spectrum and the extracted electrical characteristics of HEK cells exposed during 72 h to a 0.5 T uniform static magnetic field with those of unexposed cells. SMF potential effects on HEK proliferation kinetics and cell morphology were also assessed by using the trypan blue exclusion method and scanning electron microscopy, respectively. At last, no significant differences were observed between control and exposed HEK cells concerning electrical properties, growth, and morphology.

PMID:
23601327
PMCID:
PMC3628304
DOI:
10.1016/j.bpj.2013.01.063
[Indexed for MEDLINE]
Free PMC Article
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