Send to

Choose Destination
Trends Biotechnol. 2013 Jun;31(6):375-83. doi: 10.1016/j.tibtech.2013.03.004. Epub 2013 Apr 17.

Site-directed nucleases: a paradigm shift in predictable, knowledge-based plant breeding.

Author information

The European Food Safety Authority-EFSA, Via Carlo Magno 1A, Parma, Italy.


Conventional plant breeding exploits existing genetic variability and introduces new variability by mutagenesis. This has proven highly successful in securing food supplies for an ever-growing human population. The use of genetically modified plants is a complementary approach but all plant breeding techniques have limitations. Here, we discuss how the recent evolution of targeted mutagenesis and DNA insertion techniques based on tailor-made site-directed nucleases (SDNs) provides opportunities to overcome such limitations. Plant breeding companies are exploiting SDNs to develop a new generation of crops with new and improved traits. Nevertheless, some technical limitations as well as significant uncertainties on the regulatory status of SDNs may challenge their use for commercial plant breeding.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center