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J Lipid Res. 2013 Jul;54(7):2010-22. doi: 10.1194/jlr.D033399. Epub 2013 Apr 17.

In vivo hepatic lipid quantification using MRS at 7 Tesla in a mouse model of glycogen storage disease type 1a.

Author information

1
Université de Lyon, CREATIS, CNRS UMR 5220, Inserm U1044, INSA-Lyon, Université Lyon 1, Villeurbanne, France.

Abstract

The assessment of liver lipid content and composition is needed in preclinical research to investigate steatosis and steatosis-related disorders. The purpose of this study was to quantify in vivo hepatic fatty acid content and composition using a method based on short echo time proton magnetic resonance spectroscopy (MRS) at 7 Tesla. A mouse model of glycogen storage disease type 1a with inducible liver-specific deletion of the glucose-6-phosphatase gene (L-G6pc(-/-)) mice and control mice were fed a standard diet or a high-fat/high-sucrose (HF/HS) diet for 9 months. In control mice, hepatic lipid content was found significantly higher with the HF/HS diet than with the standard diet. As expected, hepatic lipid content was already elevated in L-G6pc(-/-) mice fed a standard diet compared with control mice. L-G6pc(-/-) mice rapidly developed steatosis which was not modified by the HF/HS diet. On the standard diet, estimated amplitudes from olefinic protons were found significantly higher in L-G6pc(-/-) mice compared with that in control mice. L-G6pc(-/-) mice showed no noticeable polyunsaturation from diallylic protons. Total unsaturated fatty acid indexes measured by gas chromatography were in agreement with MRS measurements. These results showed the great potential of high magnetic field MRS to follow the diet impact and lipid alterations in mouse liver.

KEYWORDS:

hepatic fatty acid quantification and composition; magnetic resonance spectroscopy; steatosis

PMID:
23596325
PMCID:
PMC3679402
DOI:
10.1194/jlr.D033399
[Indexed for MEDLINE]
Free PMC Article

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