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Int Forum Allergy Rhinol. 2013 Aug;3(8):630-5. doi: 10.1002/alr.21168. Epub 2013 Apr 16.

House dust mite allergen Der p 1 effects on sinonasal epithelial tight junctions.

Author information

1
Department of Otolaryngology, Emory University, Atlanta, GA 30308, USA.

Abstract

BACKGROUND:

Epithelial permeability is highly dependent upon the integrity of tight junctions, which are cell-cell adhesion complexes located at the apical aspect of the lateral membrane of polarized epithelial cells. We hypothesize that sinonasal epithelial exposure to Der p 1 house dust mite antigen decreases expression of tight junction proteins (TJPs), representing a potential mechanism for increased permeability and presentation of antigens across the sinonasal epithelial layer.

METHODS:

Confluent cultured primary human sinonasal epithelial cells were exposed to recombinant Der p 1 antigen vs control, and transepithelial resistance measurements were performed over 24 hours. Antibody staining for a panel of TJPs was examined with immunofluorescence/confocal microscopy and Western blotting. Tissue for these experiments was obtained from 4 patients total.

RESULTS:

Der p 1 exposed sinonasal cells showed a marked decrease in transepithelial resistance when compared to control cells. In addition, results of Western immunoblot and immunofluorescent labeling demonstrated decreased expression of TJPs claudin-1 and junction adhesion molecule-A (JAM-A) in Der p 1-exposed cultured sinonasal cells vs controls.

CONCLUSION:

Der p 1 antigen exposure decreases sinonasal epithelium TJP expression, most notably seen in JAM-A and claudin-1 in these preliminary experiments. This decreased TJP expression likely contributes to increased epithelial permeability and represents a potential mechanism for transepithelial antigen exposure in allergic rhinitis.

KEYWORDS:

allergic rhinitis; claudin-1; epithelium; house dust mite; junction adhesion molecule; rhinitis; sinonasal; tight junction

PMID:
23592402
PMCID:
PMC3782710
DOI:
10.1002/alr.21168
[Indexed for MEDLINE]
Free PMC Article
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