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Chembiochem. 2013 May 27;14(8):934-7. doi: 10.1002/cbic.201300102. Epub 2013 Apr 16.

Widening the pH activity profile of a fungal laccase by directed evolution.

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1
Department of Biocatalysis, Institute of Catalysis, CSIC, Marie Curie St. 2, 28049 Madrid, Spain.

Abstract

Unnatural selection: A fungal laccase was tailored by directed evolution to be active at neutral/alkaline pH. After five generations, the final mutant showed a broader pH profile while retaining 50 to 80 % of its activity at neutral pH.

PMID:
23592228
DOI:
10.1002/cbic.201300102
[Indexed for MEDLINE]
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