Format

Send to

Choose Destination
Biosens Bioelectron. 2013 Sep 15;47:566-73. doi: 10.1016/j.bios.2013.03.041. Epub 2013 Mar 29.

Direct visualization of the quadruplex structures in human chromosome using FRET: application of quadruplex stabilizer and duplex-binding fluorophore.

Author information

1
Graduate Institute of Biomedical Engineering, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan, ROC.

Abstract

The G-quadruplex structures in the telomere of a chromosome can not only protect the internal chromosome sequences by preventing the improper activation of DNA-damage-response pathways but also become targets for cancer treatments. In this manuscript, we wish to prove the existence of G-quadruplex structure formation, rather than G-quadruplex sequence, in chromosome of human cancer cells. Based on our studies, the fluorescent mapping of G-quadruplex structures in the chromosome is possible with the combination of G-quadruplex targeting fluorophore (BMVC, 3, 6-bis-(1-methyl-4-vinylpyridinium)-carbazole diiodide) and duplex-binding fluorophores (Hoechst or propidium iodide). By means of an applicable incubation time between cell cycle period and proper staining procedure to the chromosome, FRET (fluorescence resonance energy transfer) between G-quadruplex targeting fluorophore and duplex-binding fluorophore can increase the signal contrast of the fluorescent color and the fluorescent mapping of quadruplex structures can be easily observed using fluorescence microscopy. These observations are further supported by basic spectral analysis, titration binding assay, gel electrophoresis binding competition assay and confocal microscopy.

PMID:
23591020
DOI:
10.1016/j.bios.2013.03.041
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center