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PLoS One. 2013 Apr 9;8(4):e61194. doi: 10.1371/journal.pone.0061194. Print 2013.

Cobalamin analogues in humans: a study on maternal and cord blood.

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1
Department of Clinical Biochemistry, Aarhus University Hospital, Aarhus, Denmark. torehard@rm.dk

Abstract

BACKGROUND:

Haptocorrin (HC) carries cobalamin analogues (CorA), but whether CorA are produced in the body is unknown. All cobalamins (Cbl) to the foetus are delivered by the Cbl-specific protein transcobalamin (TC), and therefore analysis of cord serum for CorA may help to clarify the origin of CorA.

METHODS:

HC-CorA were quantified in paired samples of cord serum from newborns and serum from mothers (n = 69).

RESULTS:

The CorA-concentration was higher in cord serum (median = 380, range: 41-780 pmol/L) than in serum from the mothers (median = 160, range: 64-330 pmol/L), (p<0.0001). HPLC-analysis showed CorA-peaks with retention times of 13.5, 14,5 and 16.5 min in samples from both the mother and cord serum. The peak with retention time 16.5 min constituted 24% (mother) and 45% (cord serum) of the total amount CorA, and eluted as does dicyanocobinamide.

CONCLUSION:

Our results support that CorA in the human body are derived from Cbl.

PMID:
23585880
PMCID:
PMC3621755
DOI:
10.1371/journal.pone.0061194
[Indexed for MEDLINE]
Free PMC Article
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