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Appl Environ Microbiol. 2013 Jul;79(13):4149-53. doi: 10.1128/AEM.00640-13. Epub 2013 Apr 12.

Construction of mobilizable mini-Tn7 vectors for bioluminescent detection of gram-negative bacteria and single-copy promoter lux reporter analysis.

Author information

1
Department of Microbiology, Immunology, and Cancer Biology, University of Virginia Health System, Charlottesville, VA, USA.

Abstract

We describe the construction of mini-Tn7-based broad-host-range vectors encoding lux genes as bioluminescent reporters. These constructs can be mobilized into the desired host(s) by conjugation for chromosomal mini-Tn7-lux integration and are useful for localization of bacteria during infections or for characterizing regulation of promoters of interest in Gram-negative bacteria.

PMID:
23584769
PMCID:
PMC3697578
DOI:
10.1128/AEM.00640-13
[Indexed for MEDLINE]
Free PMC Article
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